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A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs) were stained with propidium iodide (A) or propidium iodide and Viobility 488/520 Fixable Dye (B) and analyzed by flow cytometry using the MACSQuant®
A) A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs), either unstained (black line) or stained with Viobility 488/520 Fixable Dye (purple line), were analyzed by flow cytometry using the MACSQuant Analyzer.
B) Two samples of human PBMCs (treated as above) were stained with Viobility 405/452 Fixable Dye. One sample was fixed, using the Inside Stain Kit (purple line), the other sample was not fixed (black line). Cells were analyzed by flow cytometry using the MACSQuant Analyzer.
Jurkat cells were treated with 1 µM of Apoptosis-inducing Staurosporin prior to staining with Viobility 488/520 Fixable Dye. Figure A shows untreated cells, Figure B shows Staurosporin-treated cells.
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