Clone REAL163 is an antibody fragment derived from the full CD62L antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL163 recognizes the human CD62L antigen, a 74 kDa single-pass type I membrane protein which is also known as L-selectin, LECAM-1, or LAM-1. CD62L is a member of the selectin family of cell surface molecules and binds a series of glycoproteins including CD34, GlyCAM-1, and MAdCAM-1. Most hematopoietic cells express CD62L, including peripheral blood B cells, T cells, monocytes, granulocytes, thymocytes, and some myeloid cells from bone marrow. Expression on resting naive, central memory, and some effector memory T cells regulates their migration via endothelial venules to peripheral lymph nodes and Peyer’s patches. The CD62L antigen also contributes to the recruitment of leukocytes from the blood to areas of inflammation. Disruption of CD62L expression has detrimental effects on T cell migration and immune responses. Antigenic activation in the lymph node leads first to rapid CD62L shedding by protease cleavage and then to transcriptional suppression.
Always use fresh material for immunofluorescent staining of CD62L
+
cells. For optimal results, the cells should not be older than 8–12 hours. Keep cells continuously cold. CD62L-expression may be rapidly lost due to shedding.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative Names

L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR

Technical specifications

  • Antigen: CD62L
  • Clone: REAL163
  • Isotype control: REAL Control
  • Alternative names of antigen: L-selectin, LAM-1, LECAM-1, Leu-8, TQ1, gp90-MEL, Lnhr, LSEL, Lyam1, PLNHR
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
    , baboon
  • Distribution of antigen: B cells, T cells, monocytes, granulocytes, thymocytes, myeloid cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioGreen
  • Selected References

    1. Ivetic, A. (2013) Signals regulating L-selectin-dependent leucocyte adhesion and transmigration. Int. J. Biochem. Cell Biol. 45(3): 550-555
    2. Telen, M. J. (2014) Cellular adhesion and the endothelium: E-selectin, L-selectin, and pan-selectin inhibitors. Hematol. Oncol. Clin. North Am. 28(2): 341-354
    3. McEver, R. P. (2015) Selectins: initiators of leucocyte adhesion and signalling at the vascular wall. Cardiovasc. Res. 107(3): 331-339
Product options: 12

Product information

Size
Order no.
Price

REAlease CD62L-FITC, human

  • REAL163
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-251
EUR  80,00

REAlease CD62L-FITC, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-077
EUR  200,00

REAlease CD62L-PE, human

  • REAL163
  • 1:50
  • For research use only
for 30
tests
(1)
130-117-840
EUR  110,00

REAlease CD62L-PE, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-117-728
EUR  265,00

REAlease CD62L-APC, human

  • REAL163
  • 1:50
  • For research use only
for 30
tests
(1)
130-112-262
EUR  110,00

REAlease CD62L-APC, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-112-095
EUR  265,00

REAlease CD62L-VioGreen, human

  • REAL163
  • 1:50
  • For research use only
for 30
tests
(1)
130-115-554
EUR  130,00

REAlease CD62L-VioGreen, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-115-436
EUR  315,00

REAlease CD62L-FITC Kit, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-122-714
EUR  265,00

REAlease CD62L-PE Kit, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-122-715
EUR  320,00

REAlease CD62L-APC Kit, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-122-716
EUR  320,00

REAlease CD62L-VioGreen Kit, human

  • REAL163
  • 1:50
  • For research use only
for 100
tests
(1)
130-122-717
EUR  330,00