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Splenocytes from LOU rats were stained with CD8a antibodies or with the corresponding REA Control antibodies (left images) as well as with CD4 (Domain 2) antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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