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Splenocytes from C57BL/6 mice were stained with CD26 antibodies or with the corresponding isotype control antibodies (left images) as well as with CD3ε antibodies. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.