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Splenocytes from Lewis rats, either left unstimulated (left image) or stimulated with 3 µg/mL conanavalin A for 48 hours, were stained with CD134 (OX40) antibodies as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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