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Human peripheral blood mononuclear cells (PBMCs) were stained with CD133/2 antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD34 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.