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Lymph nodes from C57BL/6 mice were stained with Anti-TCR Vβ12 antibodies or with the corresponding REA Control antibodies (left images) as well as with CD3ε antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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