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Mouse bone marrow cells were stained with Anti-Sca-1 antibodies conjugated to FITC (A), APC (B), PE (C), or biotin (D) and analyzed by flow cytometry. Cells stained with Anti-Sca-1-Biotin were additionally stained with Anti-Biotin-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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