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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left peak) or stimulated with 50 nM Phorbol 12-myristate 13-acetate (PMA) for 30 minutes at 37 °C, were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were stained with Anti-S6 pS240 antibodies and analyzed by flow cytometry using the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.