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Mouse thymocytes were stained with CD4-FITC. Cells were then fixed and permeabilized using the FoxP3 Staining Buffer Set. Cells were stained with Anti-RORγ (t) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. The specificity of the conjugated antibodies is confirmed by blocking the binding to ligand, using pure unconjugated antibodies (control: left images). Cell debris were excluded from the analysis based on scatter signals.
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