Anti-PLC-γ2 pY759 antibodies, human

Anti-PLC-γ2 pY759 antibodies, human

Clone: REA341
Clone REA341 recognizes the phosphoinositide phospholipase C-gamma-2 (PLC-γ2) antigen phosphorylated at tyrosine 759 (pY759). PLC-γ isoforms are mainly regulated through receptors with intrinsic tyrosine kinase activity (e.g. growth factor receptors) or receptors (such as B and T cell antigen receptors) that are linked to the activation of nonreceptor tyrosine kinases through a complex signaling network. The two isoforms of PLC-γ have distinct tissue distributions. Whereas PLC-γ1 is expressed ubiquitously, the pattern of expression of PLC-γ2 is characterized by high levels in cells of hematopoietic origin. PLC-γ2 is a transmembrane signaling enzyme that catalyzes the conversion of phosphatidyl inositol biphosphate to inositol triphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. It has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B-lymphocytes. PLC-γ2 is regulated through activation of tyrosine kinase-linked receptors. Phosphorylation of PLC-γ2 at tyrosines 753 and 759 leads to activation of the enzyme. Homozygous disruption of PLC-γ2 results in functional and signaling disorders in a subset of cell types including B cells, platelets, and mast cells.
Additional information: Clone REA341 displays negligible binding to Fc receptors.

Alternative names

PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2

Technical specifications

  • Antigen: PLC-γ2 pY759
  • Clone: REA341
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2
  • Entrez Gene ID: 5336
  • Molecular mass of antigen [kDa]: 148
  • Distribution of antigen: ubiquitous, hematopoietic stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770
  • Selected references

    1. Ombrello, M. J. et al. (2012) Cold urticaria, immunodeficiency, and autoimmunity related to PLCG2 deletions N. Engl. J. Med. 366(4): 330-338
    2. Rodriguez, R. et al. (2001) Tyrosine residues in phospholipase Cgamma 2 essential for the enzyme function in B-cell signaling J. Biol. Chem. 276(51): 47982-47992
    3. Ozdener, F. et al. (2002) Activation of phospholipase Cgamma2 by tyrosine phosphorylation Mol. Pharmacol. 62(3): 672-679
Product options: 6
for 30
tests
(1)
EUR  110,00
for 100
tests
(1)
EUR  265,00
for 30
tests
(1)
EUR  110,00
for 100
tests
(1)
EUR  265,00
for 30
tests
(1)
EUR  120,00
for 100
tests
(1)
EUR  290,00

Related products

5 products available | view all