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Fixed and permeabilized human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Perforin antibodies as well as with CD8 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed on lymphocytes according to scatter properties. Cell debris and dead cells were excluded from the analysis based on scatter signals and in a fluorescence 3 versus fluorescence 4 dot plot.
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