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Peritoneal cells were isolated from a BALB/c mouse injected with thioglycollate five days before. Adherent cells were stimulated with LPS for six hours. After two hours, brefeldin A was added. The cells were harvested and intracellularly stained with Anti-IL-6 and with CD115-APC or -PE. IL-6 staining controls (left images) were done by incubating cells with 100 μg/mL of Anti-IL-6 pure – functional grade antibody, prior to staining. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Gating was performed according to forward scatter and side scatter properties of the cells.
Anti-IL-6-PE staining control
Anti-IL-6-APC staining control
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