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Miltenyi Biotec distribution:
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with CpG A (2216) for seven hours. After three hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-α antibodies as well as with CD303 (BDCA-2) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.