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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse spleen cells were incubated with or without PMA/ionomycin for 6 hours. After 2 hours, brefeldin A was added. Cells were then fixed, permeabilized, and intracellularly stained with Anti-GM‑CSF antibodies and CD4‑FITC and CD154-APC or CD154-PE and CD45R (B220)‑PerCP and analyzed by flow cytometry using the MACSQuant®
Analyzer. The unstimulated controls can be seen in the left image. Gating was performed according to CD4 expression and side scatter properties of the cells. B cells and cell debris were excluded from the analysis in a fluorescence 3 versus fluorescence 4 dot plot.