Anti-Fetal Hemoglobin antibodies, human

Anti-Fetal Hemoglobin antibodies, human

Clone: REA533 | Dilution: 1:10
Clone REA533 recognizes the human fetal hemoglobin (HbF) antigen, a form of hemoglobin present during fetal development. The hemoglobin molecule is a tetramer composed of two subunits of α-like globin peptide chains and two subunits of the β-like globin peptides, along with heme moieties necessary for this molecule´s oxygen-carrying capacity. When production of the first enucleated definitive erythrocytes commences from stem and progenitor cells in the fetal liver, the predominant β-like globin molecule produced is γ-globin. The γ-globin chains combine with adult α-globin chains into a stable tetramer forming HbF. Shortly after the time of birth there is a switch from predominant expression of HbF to adult hemoglobin (HbA), which is mediated by a transcriptional switch in definitive erythroid progenitors from γ- to β-globin. The synthesis of HbF is normally reduced to very low levels of less than 0.6% of the total hemoglobin in adults. HbF levels may be increased in adults in a number of inherited and acquired disorders. The clinical significance of these conditions with raised HbF relates to their interaction in disorders such as sickle cell disease and β-thalassemia in which raised levels of HbF can lead to considerable amelioration of disease severity.
Additional information: Clone REA533 displays negligible binding to Fc receptors.

Alternative names

HbF, α2γ2

Technical specifications

  • Antigen: Fetal Hemoglobin
  • Clone: REA533
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: HbF, α2γ2
  • Entrez Gene ID: 3039, 3040, 3047, 3048
  • Molecular mass of antigen [kDa]: 194
  • Distribution of antigen: red blood cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Rochette, J. et al. (1994) Fetal hemoglobin levels in adults. Blood 8(4): 213-224
    2. Sankaran, V. G. et al. (2008) Human fetal hemoglobin expression is regulated by the developmental stage-specific repressor BCL11A. Science 322(5909): 1839-1842
    3. Akinsheye, I. et al. (2011) Fetal hemoglobin in sickle cell anemia. Blood 118(1): 19-27
Product options: 6
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EUR  270,00
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EUR  115,00
for 100
tests
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EUR  270,00

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