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Human MCF-7 breast cancer cells were stained with Anti-ErbB-2 (CD340) antibodies or with the corresponding isotype control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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