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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left peak) or stimulated with 20 µg/mL PHA-P for three days at 37 °C and 50 ng/mL PMA for additionally 15 minutes at 37 °C. Cells were then fixed and permeabilizied using the Cell Signaling Buffer Set A and stained with Anti-ELK-1 pS383 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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