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Splenocytes from C57BL/6 mice were either left unstimulated (left image) or stimulated with CD3ε and CD28 antibodies as well as with interleukin 2 (IL-2) over night. Afterwards, PMA and ionomycin were added for two hours. Cells were then fixed and permeabilized using the FoxP3 Staing Buffer Set and stained with Anti-EGR-2 antibodies as well as with CD19 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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