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Human K-562 cells were either left untreated (right peak) or treated with 25 µM Imatinib for 2 hours at 37 °C. Cells were then fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-CrkL pY207 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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