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Jurkat cells were serum starved overnight and stimulated with 5 mM hydrogen peroxide for 15 minutes. The cells were then fixed and permeabilized with the Cell Signaling Buffer Set A and stained with Anti-c-Cbl pY774 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant®
Analyzer. Left peak represents the control. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris were excluded from the analysis based on scatter signals.
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