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Separation of human leukocyte-enriched buffy coat using CD45 MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells are stained with CD45-FITC.
Peripheral blood before separation
Enrichment of tumor cells from a mixture of PBMCs and cells of the breast cancer cell line SK-BR3 using CD45 MicroBeads.
(A) Cell mixture before separation (7×106 PBMCs were spiked with 20,000 tumor cells)
(B) Negative fraction with enriched breast cancer cells
Cell mixture before separation (7×106
PBMCs were spiked with 20,000 tumor cells)
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