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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Tumors induced by 4T1 cell line were dissociated by using the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS Octo Dissociator (# 130-095-937). CD45+
cells were depleted by using the CD45 MicroBeads, a MidiMACS™ Separator, and a LS Column. CD326 (EpCAM)+
cells were isolated by using the CD326 (EpCAM) MicroBeads, a MidiMACS Separator, and a LS Column. The cells were fluorescently stained with CD45-PE (# 130-102-596) and CD326 (EpCAM)-APC (# 130-102-234) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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