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Tumors induced by 4T1 cell line were dissociated by using the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS Octo Dissociator (# 130-095-937). CD45+
cells were depleted by using the CD45 MicroBeads, a MidiMACS™ Separator, and a LS Column. CD326 (EpCAM)+
cells were isolated by using the CD326 (EpCAM) MicroBeads, a MidiMACS Separator, and a LS Column. The cells were fluorescently stained with CD45-PE (# 130-102-596) and CD326 (EpCAM)-APC (# 130-102-234) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.