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T cells and CD19+
B cells were isolated from 5 mL of human EDTA-anticoagulated whole blood using the MACSprep™ HLA B/T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress®
Separator. The isolated cells were fluorescently stained with CD45-VioBlue, CD3-FITC, CD19-APC, and CD56-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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