CD146 MicroBead Kit, human

CD146 MicroBead Kit, human

The CD146 MicroBead Kit was developed for the isolation of CD146
cells. The kit consists of CD146 MicroBeads and FcR Blocking Reagent. The CD146 MicroBeads recognize the human CD146 antigen, also known as MUC18, MCAM, Mel-CAM, and S-Endo-1.

Background information

CD146 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily and contains five extracellular immunoglobulin (Ig)-like domains.
CD146 possesses a limited tissue expression distribution, including endothelial cells, smooth muscle cells, pericytes, follicular dendritic cells, melanoma cells, and a subpopulation of activated T lymphocytes.
cells isolated from umbilical cord
, lipoaspirate
, dental pulp, or endometrial tissue
, are described to contain cells with clonogenic fibroblastic colony (CFU-F) activity and multilineage differentiation potential, which are often called mesenchymal or marrow stromal cells (MSCs).
CD146 functions as a Ca
-independent cell adhesion molecule, which is involved in heterophilic cell-cell interactions
, and may be involved in the extravasion and/or homing of activated T cells
. CD146 expression in melanoma is also directly associated with tumor growth and metastasis.


The CD146 MicroBead Kit can be used for:
  • isolation of human mesenchymal stromal cells (MSCs) from lipoaspirate and other tissue sources,
  • isolation of endothelial cells and pericytes from human endothelial tissue,
  • isolation of human umbilical vein endothelial cells (HUVECs) and human umbilical cord perivascular cells (HUCPVCs)4 from umbilical cord vein.


LS or XS Columns.
  • Selected references

    1. Bardin, N. et al. (2001) Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion. Blood 98: 3677-3684
    2. Yan, X. et al. (2003) A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth. Blood 102: 184-191
    3. Pickl, W. F. et al. (1997) MUC18/MCAM (CD146), an activation antigen of human T lymphocytes. J. Immunol. 158: 2107-2115
    4. Baksh, D. et al. (2007) Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells 25: 1384-1392
    5. Zannettino, A. C. et al. (2008)
      Multipotential human adipose-derived stromal stem cells exhibit a perivascular phenotype
      in vitro
      in vivo
      J. Cell. Physiol. 214: 413-421
    6. Schwab and Gargett (2005) Reprod. Fertil. Dev. 17: 111-111
    7. Xie, S. et al. (1997) Expression of MCAM/MUC18 by human melanoma cell leads to increased tumor growth and metastasis. Cancer Res. 57: 2295-2303
    8. Satyamoorthy, K. et al. (2001) Mel-CAM-specific genetic suppressor elements inhibit melanoma growth and invasion through loss of gap junctional communication. Oncogene 20: 4676-4684
  • Brochures and posters

Product options: 1
for 1×
total cells
EUR  583,00

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