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PDCs were isolated from splenocytes by using the Plasmacytoid Dendritic Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells were fluorescently stained with Anti-mPDCA-1-FITC and Anti-Siglec-H-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Plasmacytoid dendritic cells
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