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Pan DCs were isolated from mouse spleen cell suspension using the Pan Dendritic Cell Isolation Kit, mouse, an LS Column, and a MidiMACS™ Separator. The cells were fluorescently stained with CD11c-APC and mPDCA-1-FITC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris, dead cells and lineage positive cells were excluded from the analysis based on scatter signals, propidium iodide fluorescence and lineage antigen expression.
Isolated total DCs
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