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Isolation of PDCs from PBMCs using the Diamond Plasmacytoid Dendritic Cell Isolation Kit II, an LD Column and a MidiMACS™ Separator, as well as a MiniMACS™ Separator and two MS Columns.
Cells were stained with CD303 (BDCA-2)-APC and CD123-PE. Like CD304 (BDCA-4/Neuropilin-1), the CD303 (BDCA-2) antigen is specifically expressed on PDCs in blood and allows their direct identification.
PBMCs before separation
Enriched PDCs after depletion of non-PDCs
Pure PDCs after positive selection with CD304 (BDCA-4/Neuropilin-1) Diamond MicroBeads
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