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memory B cells were isolated from human PBMCs using the IgM+
Memory B Cell Isolation Kit, an LD Column and a MidiMACS™ Separator, an MS Column and a MiniMACS™ Separator. Cells were fluorescently stained with Anti-IgM-APC and CD27-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
PBMCs before separation
Pre-enriched memory B cells
memory B cells
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