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cells were isolated from human PBMCs using Anti-IgD MicroBeads, an MS Column, and a MiniMACS™ Separator. Cells were fluorescently stained with CD23-PE (# 130-094-505) as well as with Anti-IgD-APC (# 130-094-553) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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