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Separation of CD3+
cells from human PBMCs using a biotinylated CD3 antibody, Anti-Biotin MicroBeads, and an MS Column.
PBMCs before separation
cells were isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a- Biotin, Anti-Biotin MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-Biotin-PE (# 130-090-756), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.
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