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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
regulatory T cells were isolated from 8 mL of human EDTA-anticoagulated whole blood using the MACSxpress®
Whole Blood Treg Isolation Kit, an LS Column, an overhead rotator, a MidiMACS™ Separator, and a MACSxpress Separator. An aliquot of the original whole blood was taken and red blood cells were lysed. Cells taken before and after separation were fluorescently stained with CD45-VioBlue®
, CD4-FITC, CD25-PE, and CD127-APC and were analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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