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Untouched pan B cells were isolated from a buffy coat using the MACSxpress®
Buffy Coat Pan B Cell Isolation Kit, a MACSmix™ Tube Rotator, and MACSxpress Separators. The isolated cells were fluorescently stained with CD19-PE, CD20-APC, and CD45-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.
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