Enrichment of CD138⁺ plasma cells for analysis of Multiple Myeloma samples

Direct isolation of CD138⁺ PCs from bone marrow or peripheral blood in 20 minutes
No density gradient centrifugation, no erythrocyte lysis
Scalable automation solutions

MACSprep Multiple Myeloma CD138 MicroBeads, human allow the isolation of CD138⁺ plasma cells (PCs) directly from bone marrow or peripheral blood without the need for gradient centrifugation or erythrocyte lysis. The minimal manipulation of sample ensures high integrity of CD138⁺ PCs, suitable for further cellular (flow cytometry), cytogenetic (FISH), or molecular analysis (sequencing, PCR, microarray) of bone marrow samples.

Basic principle

Basic principle of MACSprep™ Multiple Myeloma CD138 MicroBeads

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Three easy steps to isolate PCs directly from bone marrow or peripheral blood samples:

1. Magnetic labeling: PCs are magnetically labeled directly in the sample with MACSprep Multiple Myeloma CD138 MicroBeads.

2. Magnetic separation: The flow-through fraction, containing erythrocytes and non-target cells, can be collected as the negative fraction.

3. Elution of labeled cells: The column is removed from the separator. The retained cells are eluted as the enriched, positively selected PCs.

Video

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Video
Direct cell isolation from bone marrow or blood with the autoMACS® Pro Separator

With the autoMACS® Pro Separator, CD138⁺ PCs can be automatically isolated from bone marrow or peripheral blood samples. Take this short instrument tour and see how your target cells are being separated without the need for density gradient centrifugation or red blood cell lysis.

Data

Isolation of CD138⁺ PCs from bone marrow

CD138⁺ PCs were isolated from a blood marrow sample using MACSprep™ Multiple Myeloma CD138 MicroBeads and the autoMACS® Pro Separator.

Before separation

After separation

Before separation

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After separation

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Cells were fluorescently stained with CD138-APC, CD38-FITC, and CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.