MACSprep Multiple Myeloma CD138 MicroBeads, human allow the isolation of CD138+ plasma cells (PCs) directly from bone marrow or peripheral blood without the need for gradient centrifugation or erythrocyte lysis. The minimal manipulation of sample ensures high integrity of CD138+ PCs, suitable for further cellular (flow cytometry), cytogenetic (FISH), or molecular analysis (sequencing, PCR, microarray) of bone marrow samples.
Isolation of CD138+ PCs from bone marrow
CD138+ PCs were isolated from a blood marrow sample using MACSprep™ Multiple Myeloma CD138 MicroBeads and the autoMACS® Pro Separator.
Cells were fluorescently stained with CD138-APC, CD38-FITC, and CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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