Anti-Biotin MACSiBead Particles were loaded with CD335 (NKp46) and CD2 antibodies. NK cells were activated and expanded from PBMCs using one loaded MACSiBead particle per viable cell. Cells were cultured in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2. After 2 weeks, NK cells were incubated with K562 target cells in different ratios.
In addition, NK cells incubated in NK MACS Medium supplemented with 5% AB serum and 500 IU/mL IL-2 alone were analyzed. Specific target cell lysis by NK cells was determined after 4 hours.