Mouse SCF

Mouse SCF

SCF stands for stem cell factor, also termed c-Kit ligand. Mouse SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse SCF may be used for a variety of applications, including:
  • In vitro culture and expansion of hematopoietic progenitor cells.
  • Colony formation assays.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • Mast cell differentiation and maintenance.
  • Investigation of receptor signaling.

Alternative names

c-kit ligand, steel factor, MGF

Background information

Stem cell factor (SCF), also known as c-kit ligand, is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms, cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. Recombinant Mouse SCF corresponds to the secreted soluble form.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Proliferation of TF-1 cells
  • premium grade: ≥ 4×
    10
    4
    U/mg
  • research grade: ≥ 2×
    10
    4
    U/mg
  • Selected references

    1. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    2. Narla, A. et al. (2011) Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood 118(8): 2296-2304
    3. Rodriguez, S. et al. (2011) The SKP2 E3 ligase regulates basal homeostasis and stress-induced regeneration of HSCs. Blood 117(24): 6509-6519
    4. Velardi, E. et al. (2014) Sex steroid blockade enhances thymopoiesis by modulating Notch signaling. J. Exp. Med. 211(12): 2341-2349
    5. Kitumura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    6. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    7. Dussiau C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
    8. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    9. Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
Product options: 6
E. coli
10 µg
EUR  135,00
E. coli
25 µg
EUR  210,00
E. coli
10 µg
EUR  165,00
E. coli
25 µg
EUR  265,00
E. coli
100 µg
EUR  525,00
E. coli
1000 µg
EUR  2.220,00