Mouse IL-3 IS

Mouse IL-3 IS

IL-3 IS stands for interleukin 3 "Improved Sequence". Mouse IL-3 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-3 IS can be used for a variety of applications, including:
  • As cell culture supplement to maintain hematopoietic cells or cell lines, such as the pro-B lymphoid cell line BaF/3 or 32Dcl3 cells.
  • Induction of colony formation of hematopoietic progenitor cells in semi-solid medium, e.g. of highly purified Sca-1+ /lin- cells.
  • Pre-stimulation of hematopoietic progenitor cells for retroviral transduction prior to bone marrow transplantation.
  • In vitro differentiation studies, e.g. of mast cells.

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biologic activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 32D cells (NIBSC 91/662)
  • premium grade: ≥ 3×
    10
    7
    U/mg
  • research grade: ≥ 2×
    10
    7
    U/mg
  • Selected references

    1. Rodriguez, S. et al. (2011) The SKP2 E3 ligase regulates basal homeostasis and stress-induced regeneration of HSCs. Blood 117(24): 6509-6519
    2. Rochelle, T. et al. (2013) p210bcr-abl induces amoeboid motility by recruiting ADF/destrin through RhoA/ROCK1. FASEB J. 27(1): 123-134
Product options: 6
E. coli
10 µg
EUR  155,00
E. coli
25 µg
EUR  290,00
E. coli
10 µg
EUR  190,00
E. coli
25 µg
EUR  365,00
E. coli
100 µg
EUR  630,00
E. coli
1000 µg
EUR  2.750,00