Mouse IL-12

Mouse IL-12

IL-12 stands for interleukin 12. Mouse IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-12 can be used for a variety of applications, including:
  • In vitro activation and proliferation of NK and T cells.
  • In vitro differentiation of naive CD4+ T cells towards TH 1 cells.
  • In vitro stimulation of cytotoxic activity of NK cells and T cells.

Background information

Interleukin 12 (IL-12) is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation, and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T
H
1 rather than the T
H
2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive
in vitro
and
in vivo
.
2,3

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

  • Induction of IFN-γ secretion by mouse splenocytes
  • research grade: ≥ 6×
    10
    6
    U/mg
  • Selected references

    1. Mattner, F. et al. (1993) The interleukin-12 subunit p40 specifically inhibits effects of the interleukin-12 heterodimer. Eur. J. Immunol. 23: 2202-2208
    2. Lieschke, G.J. et al. (1997)
      Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity
      in vivo.
      Nat. Biotechnol. 15: 35-40
    3. Miller, J.M. et al. (2010)
      Vesicular stomatitis virus modified with single chain IL-23 exhibits oncolytic activity against tumor cells
      in vitro
      and
      in vivo.
      Int. J. Infereron Cytokine Mediator Res. 2010: 63-72
Product options: 3
HEK293 cells
5 µg
EUR  130,00
HEK293 cells
25 µg
EUR  400,00
HEK293 cells
100 µg
EUR  1.270,00