Human FGF-8b

Human FGF-8b

FGF-8b stands for fibroblast growth factor 8b. Human FGF-8b is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-8b can be used for a variety of applications, including:
  • Differentiation of embryonic stem cells into neutral progenitor cells.
  • Differentiation of embryonic stem cell-derived neural progenitor cells into dopaminergic neurons.
  • Survival of neural precursor cells and differentiation into astroglial cells.
  • Stimulation of osteoblast proliferation.
  • Proliferation of endothelial cells.
  • Proliferation of myogenic cells.
  • Tumor growth studies of prostate and breast cancer.
  • Protection of hippocampal cultures from oxidative stress.

Background information

Fibroblast growth factor 8 (FGF-8), originally identified as androgen-induced growth factor, is a heparin-binding protein, which has mitogenic and transforming activity. Eight secreted FGF-8 isoforms (FGF-8a to h) have been identified in mice, whereas four have been described in humans (a, b, e, and f). FGF-8b appears to be the predominant form and has been shown to have the most important oncogenic transforming capacity. FGF-8 exerts a pivotal role in embryogenesis. It is expressed during gastrulation and influences brain, limb, heart and facial development in the mouse embryo. In addition, FGF-8 was shown to stimulate osteoblast proliferation. The amino acid sequence of human FGF8-b shares 100% identity with mouse FGF-8b.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 3T3 cells
  • premium grade: ≥ 2×
    10
    4
    U/mg
  • research grade: ≥ 1×
    10
    4
    U/mg
Product options: 6
E. coli
10 µg
EUR  100,00
E. coli
25 µg
EUR  155,00
E. coli
10 µg
EUR  130,00
E. coli
25 µg
EUR  190,00
E. coli
100 µg
EUR  525,00
E. coli
1000 µg
EUR  2.220,00