Clone REAL296 is an antibody fragment derived from the full Ly-49C/I antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL296 recognizes the mouse lymphocyte antigen 49c (Ly-49C), a type II transmembrane protein which is also known as killer cell lectin-like receptor 3 (Klra3). Clone REAL296 reacts with Ly-49C of BALB, B6, NZB, C57BL, and Ly-49I of B6 mice strains. Ly-49 family members are both activating and inhibitory receptors and are mainly receptors for MHC class I molecules. Ly-49C is a cell surface molecule expressed on a subpopulation of murine natural killer (NK) cells and NK1.1 T cells that are involved in the specific rejection of H-2d or H-2f (hemopoietic histocompatibility determinant 2) bone marrow cell grafts. It transduces a negative signal to NK cells upon recognition of class I antigens. The particular allele of Ly-49C, i.e., C57BL versus BALB, determines to a large extent which class I antigen (H-2Kb for B6, Kb and H-2d for BALB/c or BALB.B) sends negative signals to Ly-49C
+ NK cells. Clone REAL296 blocks the binding of Ly-49C to MHC class I antigens of the b, d, k, and s haplotypes. The epitope recognized by REAL296 may be masked on NK cells due to interactions with MHC class I molecules.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
