Clone:
REA665
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC, MICS, IHC, IF
Alternative names:
Interleukin-2, T cell growth factor, TCGF, EDF, KHF, MAF-C I, TDF

Extended validation for IL-2 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA665
JES6-5H4++
Cells were incubated with an excess of purified unconjugated IL-2 (REA665) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for IL-2. C57BL/6 Mouse splenocytes stimulated with PdBu, Ionomycin and BrefeldinA for 5 hours were stained with Viobility 405/453 Fixable Dye before fixation for detection of dead cells followed by a staining with IL-2 antibodies and with a suitable counterstaining. As a control, IL-2 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for IL-2 Antibody, anti-mouse, REAfinity™

Overview

Clone REA665 recognizes the mouse interleukin-2 (IL-2) antigen. The T cell growth factor IL-2, mainly produced by activated CD4
+
T cells, is a central regulator of the immune response. It induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, monocytes, and oligodendrocytes. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance, and anti-inflammatory reactions.
Additional information: Clone REA665 displays negligible binding to Fc receptors.

Alternative names

Interleukin-2, T cell growth factor, TCGF, EDF, KHF, MAF-C I, TDF

Detailed product information

Technical specifications

CloneREA665
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenIL-2
Alternative names of antigenInterleukin-2, T cell growth factor, TCGF, EDF, KHF, MAF-C I, TDF
Molecular mass of antigen [kDa]17
Distribution of antigenT cells
Entrez Gene ID16183
RRIDAB_2733293, AB_2752028, AB_2751995, AB_2889698, AB_2922001, AB_2733292

Resources for IL-2 Antibody, anti-mouse, REAfinity™

Certificates

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References for IL-2 Antibody, anti-mouse, REAfinity™

Publications

  1. Matesanz, F. et al. (1992) A new cDNA sequence for the murine interleukin-2 gene. Biochim. Biophys. Acta 1132(3): 335-336
  2. Matesanz, F. et al. (1993) Existence of at least five interleukin-2 molecules in different mouse strains. Immunogenetics 38(4): 300-303
  3. Fuse, A. et al. (1984) Organization and structure of the mouse interleukin-2 gene. Nucleic Acids Res. 12(24): 9323-9331

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