PDGF-BB IS stands for platelet derived growth factor BB "Improved Sequence". Human PDGF-BB IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. Human PDGF-BB IS possesses the same properties as naturally occurring PDFG-BB.

Data and images for Human PDGF-BB IS

Figures

Figure 1

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Human PDGF-BB IS biological activity.
The biological activity of Human PDGF-BB IS is determined by proliferation assay using 3T3 cells. Activity of Human PDGF-BB IS, premium grade, (red line) was compared to another commercially available product (black line).

Figure 1

Human PDGF-BB IS biological activity.
The biological activity of Human PDGF-BB IS is determined by proliferation assay using 3T3 cells. Activity of Human PDGF-BB IS, premium grade, (red line) was compared to another commercially available product (black line).

Figure 2

View details
Human PDGF-BB IS biological activity.
Activity of Human PDGF-BB IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 2

Human PDGF-BB IS biological activity.
Activity of Human PDGF-BB IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

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SDS-PAGE of Human PDGF-BB IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human PDGF-BB IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

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Mass spectrometry analysis (ESI-MS) of Human PDGF-BB IS, premium grade. The peak corresponds to the calculated molecular mass of 24317 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human PDGF-BB IS, premium grade. The peak corresponds to the calculated molecular mass of 24317 Da.

Specifications for Human PDGF-BB IS

Overview

PDGF-BB IS stands for platelet derived growth factor BB "Improved Sequence". Human PDGF-BB IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. Human PDGF-BB IS possesses the same properties as naturally occurring PDFG-BB.

Applications

Human PDGF-BB IS can be used for a variety of applications, including:
  • MSC culture and investigation of MSC development.
  • Studies of angiogenesis.

Detailed product information

Background information

Platelet-derived growth factor-BB (PDGF-BB) is a dimeric protein composed by two identical B subunits, it belongs to the PDGF family together with the two receptors PDGFRα and PDGFRβ. PDGF-BB binds PDGFRβ with the highest affinity. It regulates mesenchymal cell growth, morphogenesis, and migration, especially during angiogenesis and glial cell proliferation. A dysregulation of PDGF was shown in diseases such as diabetes, and in deficit of bone formation and reparation. Human PDGF-BB IS is an engineered PDGF-BB variant retaining the same biological properties as natural accurring PDGF-BB.

Biological activity

  • Proliferation of 3T3 cells (NIBSC 94/728)
  • research grade: ≥ 5×
    10
    5
    IU/mg
  • premium grade: ≥ 1×
    10
    6
    IU/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human PDGF-BB IS

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human PDGF-BB IS

Publications

  1. Wilhelm, A. et al. (2016) Interaction of TWEAK with Fn14 leads to the progression of fibrotic liver disease by directly modulating hepatic stellate cell proliferation J. Pathol. 239(1): 109-121
  2. Moench, R. et al. (2016)
    Exclusive inhibition of PI
    3
    K/Akt/mTOR signaling is not sufficient to prevent PDGF-mediated effects on glycolysis and proliferation in colorectal cancer
    Oncotarget. 7(42): 68749-68767
  3. Hagmann, S. et al. (2013) Different culture media affect growth characteristics, surface marker distribution and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells BMC Musculoskelet Disord 14: 223
  4. Dilasser, F. et al. (2021) Essential role of smooth muscle Rac1 in severe asthma-associated airway remodelling Ther Clin Risk Manag 76(4): 326-334
  5. Shepherd, E. L. et al. (2020) Ketohexokinase inhibition improves NASH by reducing fructose-induced steatosis and fibrogenesis JHEP Rep 3(2): 100217

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