IL-8 stands for interleukin 8. Human IL-8 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human IL-8

Figures

Figure 1

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Human IL-8 activity assay.
The biological activity of Human IL-8, premium grade, was determined by migration assay using neutrophil granulocytes.

Figure 1

Human IL-8 activity assay.
The biological activity of Human IL-8, premium grade, was determined by migration assay using neutrophil granulocytes.

Figure 2

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SDS-PAGE of Human IL-8, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 2

SDS-PAGE of Human IL-8, premium grade
under reducing (R) and non-reducing (NR) conditions.

Specifications for Human IL-8

Overview

IL-8 stands for interleukin 8. Human IL-8 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-8 can be used for a variety of applications, including:
  • Study of leukocyte chemotaxis.
  • Investigation of CXCR1 and CXCR2 signaling pathway.

Detailed product information

Background information

Interleukin 8 (IL-8), also known as CXCL8, is a pro-inflammatory molecule belonging to the CXC chemokine family. The precursor form of IL-8 is cleaved into several mature isoforms (including 72 aa and 77 aa) and is secreted by several type of cells, especially monocytes and endothelial cells, upon pro-inflammatory stimulation. IL-8 signals through the CXCR1 and CXCR2 receptors, chemoattracts and activates granulocytes, predominantly neutrophils, T cells, and plays also an angiogenic role. Overexpression of IL-8 can lead to epithelial damage and injury of the airway tissues.

Biological activity

  • Migration assay using neutrophils (NIBSC code 89/520)
  • research grade: ≥ 8×
    10
    5
    IU/mg
  • premium grade: ≥ 1×
    10
    6
    IU/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-8

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-8

Publications

  1. Lim, C. H. et al. (2017) Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo Sci Rep 7(1): 11201
  2. Sharma, K. et al. (2021) Dynamic persistence of UPEC intracellular bacterial communities in a human bladder-chip model of urinary tract infection Elife 10: e66481

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