Human IFN-γ1b

Name: Human IFN-γ1b
Availability: InStock

Human IFN-γ1b

Alternative names:

IFN-γ

Product data and images for Human IFN-γ1b

Figures

Figure 1

**Human IFN-γ1b activity assay.**
The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).

Figure 1

Human IFN-γ1b activity assay.

The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ1b, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

**SDS-PAGE of Human IFN-γ1b, premium grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IFN-γ1b, premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 3

**Human IFN-γ1b biological activity.**
Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human IFN-γ1b biological activity.

Activity of Human IFN-γ1b, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IFN-γ1b, premium grade. The peak corresponds to the calculated molecular mass of 16334 Da.

Specifications for Human IFN-γ1b

Overview

IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IFN-γ1b can be used for a variety of applications, including:

Stimulation of monocytes, macrophages, and microglia.
In vitro maturation of Mo-DCs.
Apoptosis and cell growth inhibition assays.
Adhesion and migration assays.

Alternative names

IFN-γ

Detailed product information

Background information

IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.

Biological activity

Proliferation of HT-29 cells (NIAID Gxg01-902-535)
research grade: ≥ 1×
10
⁷
IU/mg
premium grade: ≥ 2×
10
⁷
IU/mg

(Typical specific activity: ≥ 5×
10
⁷
IU/mg
)
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IFN-γ1b

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human IFN-γ1b

Publications

Wong, V. L. et al. (1989) Growth-inhibitory activity of interferon-beta against human colorectal carcinoma cell lines. Int. J. Cancer 43: 526-530
Jones, S. P. et al. (2015) Expression of the Kynurenine Pathway in Human Peripheral Blood Mononuclear Cells: Implications for Inflammatory and Neurodegenerative Disease. PLoS One 10(6): e0131389
Fan, X.-Y. et al. (2017) All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion. Sci Rep 7(1): 16481
Leuci, V. et al. (2018) CD44v6 as innovative sarcoma target for CAR-redirected CIK cells. Oncoimmunology 7(5): e1423167
Mesiano, G. et al. (2018) Cytokine Induced Killer cells are effective against sarcoma cancer stem cells spared by chemotherapy and target therapy. Oncoimmunology 7(11): e1465161
Ding, X. et al. (2015) Cellular immunotherapy as maintenance therapy prolongs the survival of the patients with small cell lung cancer. J Transl Med. 13: 158
Niu, C. et al. (2015) In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells. BMC Immunol. 16: 61
Graciotti, M. et al. (2020) Deciphering the Mechanisms of Improved Immunogenicity of Hypochlorous Acid-Treated Antigens in Anti-Cancer Dendritic Cell-Based Vaccines. Vaccines (Basel) 8(2): 271

Data and images