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Mouse spleen cells were incubated with or without PMA/ionomycin for 6 hours. After 2 hours, brefeldin A was added. Cells were then fixed, permeabilized, and intracellularly stained with Anti-GM‑CSF antibodies and CD4‑FITC and CD154-APC or CD154-PE and CD45R (B220)‑PerCP and analyzed by flow cytometry using the MACSQuant® Analyzer. The unstimulated controls can be seen in the left image. Gating was performed according to CD4 expression and side scatter properties of the cells. B cells and cell debris were excluded from the analysis in a fluorescence 3 versus fluorescence 4 dot plot.
Clone | MP1-22E9 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2aκ |
Isotype control | Isotype Control Antibody, rat IgG2a |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | GM-CSF |
Alternative names of antigen | CSF2, Csfgm, GM-CSF, MGI-IGM |
Molecular mass of antigen [kDa] | 14 |
Entrez Gene ID | 12981 |
RRID | AB_2651854, AB_2651855, AB_2651850 |
Clone | MP1-22E9 |
---|---|
Clonality | monoclonal |
Isotype | rat IgG2aκ |
Isotype control | Isotype Control Antibody, rat IgG2a |
Host | rat |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | mouse |
Antigen | GM-CSF |
Alternative names of antigen | CSF2, Csfgm, GM-CSF, MGI-IGM |
Molecular mass of antigen [kDa] | 14 |
Entrez Gene ID | 12981 |
RRID | AB_2651854, AB_2651855, AB_2651850 |
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