Clone:
REA296
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74

Specifications for CLIP Antibody, anti-human, REAfinity™

Overview

Clone REA296 recognizes MHC class II-associated invariant chain (Ii)-derived peptide (CLIP) complexes. MHC class II αβ heterodimers associate early during biosynthesis with a type II membrane protein, the invariant chain (Ii). The invariant chain serves as a chaperone for MHC II molecules and mediates trafficking to the endosomal pathway. In the endosomal pathway Ii is sequentially degraded, leaving a residual CLIP in the peptide-binding groove of MHC II. In presence of antigen peptide fragments, HLA-DM then binds to the MHC II molecule, releasing CLIP and allowing peptides to bind. REA296 detects HLA class II-positive cells which have impaired HLA-DM activity, and tumor cells that have escaped immuno-surveillance by CD4-positive T cells.
Additional information: Clone REA296 displays negligible binding to Fc receptors.

Alternative names

Class II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74

Detailed product information

Technical specifications

CloneREA296
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCLIP
Alternative names of antigenClass II-associated invariant chain peptide, DHLAG, HLADG, II, Ia-GAMMA, CD74
Molecular mass of antigen [kDa]34
Distribution of antigenB cells, dendritic cells, macrophages, monocytes
Entrez Gene ID972
RRIDAB_2651444, AB_2651445, AB_2651446, AB_2651447, AB_2651448, AB_2651440, AB_2651441, AB_2651442, AB_2651443, AB_2651439

Resources for CLIP Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CLIP Antibody, anti-human, REAfinity™

Publications

  1. van Bergen, J. et al. (1997) Efficient loading of HLA-DR with a T helper epitope by genetic exchange of CLIP. Proc. Natl. Acad. Sci. U.S.A. 94(14): 7499-7502
  2. Wälchli, S. et al. (2014) Invariant chain as a vehicle to load antigenic peptides on human MHC class I for cytotoxic T-cell activation. Eur. J. Immunol. 44(3): 774-784
  3. Bakke, O. et al. (1990) MHC class II-associated invariant chain contains a sorting signal for endosomal compartments. Cell 63(4): 707-716

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