CD80 Antibody, anti-mouse

Name: CD80 Antibody, anti-mouse
Availability: InStock

CD80 Antibody, anti-mouseExtended Validation

Clone:

16-10A1

Type of antibody:

Primary antibodies

Isotype:

hamster IgG2κ

Applications:

FC

Alternative names:

B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Flow cytometry applications forCD80 Antibody, anti-mouse

APC
Biotin
FITC
PE
PE-Vio 770

Conjugate:

APC

Recommended dilution:

1:10

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-102-884, 130-102-584

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:10)

Description:

LPS activated mouse splenocytes were stained with CD80 antibodies conjugated to FITC, PE, APC, PE-Vio770 and Biotin, as well as with CD11c antibodies conjugated to FITC or PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD80-Biotin were stained with Anti-Biotin-PE as well. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of CD80-PE-Vio770.

Extended validation for CD80 Antibody, anti-mouse

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD80. C57BL/6 splenocytes were stimulated with 100ng/ml LPS for over night at 37°C and were stained with CD80 antibodies and with a suitable counterstaining. As a control, CD80 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD80 (16-10A1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD80 (16-10A1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD80 Antibody, anti-mouse

Overview

The monoclonal antibody 16-10A1 recognizes mouse CD80 also known as B7, B7-1 or Ly-53. The 55 kDa member of the Ig superfamily is expressed on activated B cells, macrophages and dendritic cells. The interaction of CD80 and CD152 (CTLA-4) or CD28 induces T cell proliferation and cytokine production.

Alternative names

B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1

Detailed product information

Technical specifications

Clone	16-10A1
Clonality	monoclonal
Isotype	hamster IgG2κ
Host	hamster
Type of antibody	Primary antibodies
Species	mouse, other
Cross-reactivity	dog
Antigen	CD80
Alternative names of antigen	B7-1, Cd28l, Ly-53, Ly53, MIC17, TSA1, B7, BB1
Molecular mass of antigen [kDa]	30
Distribution of antigen	B cells, dendritic cells, macrophages, monocytes, Langerhans cells, T cells
Entrez Gene ID	12519
RRID	AB_2659264, AB_2659265, AB_2659266, AB_2659267, AB_2659268, AB_2659269, AB_2659270, AB_2659271, AB_2659272, AB_2659273

Resources for CD80 Antibody, anti-mouse

Certificates

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Reviews for CD80 Antibody, anti-mouse

Staining CD80 in Skin

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CD80-PE, mouse (130-102-613)

It works well to detect targeted cells in the skin.

Staining CD80 in Skin

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CD80-PE, mouse (130-102-883)

It works well to detect targeted cells in the skin.

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References for CD80 Antibody, anti-mouse

Publications

Bluestone, J. A. et al. (1995) New perspectives of CD28-B7-mediated T cell costimulation. Immunity 2(6): 555-559
Hathcock, K. S. et al. (1994) Comparative analysis of B7-1 and B7-2 costimulatory ligands: expression and function. J. Exp. Med. 180(2): 631-640
Harlan, D. M. et al. (1994) Mice expressing both B7-1 and viral glycoprotein on pancreatic beta cells along with glycoprotein-specific transgenic T cells develop diabetes due to a breakdown of T-lymphocyte unresponsiveness. Proc. Natl. Acad. Sci. U.S.A. 91: 3137-3141
Razi-Wolf, Z. et al. (1992) Expression and function of the murine B7 antigen, the major costimulatory molecule expressed by peritoneal exudate cells. Proc. Natl. Acad. Sci. U.S.A. 89: 4210 -4214

Applications

Extended validation