CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8
+
T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies
1
, and spleen.

Data and images for CD8 MicroBeads, human

Figures

Figure 1

Separation of CD8
+
cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.
PBMCs before separation
CD8
-
cells
View details

Figure 1

Separation of CD8
+
cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.
View details

Figure 1

Separation of CD8
+
cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.
CD8
+
cells
View details

Figure 1

Separation of CD8
+
cells from PBMCs using CD8 MicroBeads, an LS Column, and a MidiMACS™ Separator.

Figure 2

View details
Scanning electron micrograph of CD8
+
T cells isolated using CD8 MicroBeads (courtesy of Prof. Peter Groscurth, Institute of Anatomy, University of Zürich, Switzerland).

Figure 2

Scanning electron micrograph of CD8
+
T cells isolated using CD8 MicroBeads (courtesy of Prof. Peter Groscurth, Institute of Anatomy, University of Zürich, Switzerland).

Specifications for CD8 MicroBeads, human

Overview

CD8 MicroBeads were developed for the positive selection or depletion of human cytotoxic CD8
+
T cells from different cell sources. The most commonly used cell sources are peripheral blood and cord blood. In addition, cytotoxic T cells have also been isolated from lymph nodes, thymus, skin biopsies
1
, and spleen.

Detailed product information

Background information

The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8
+
cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications

Isolation or depletion of CD8
+
cytotoxic T cells is performed in many different research fields such as infectious diseases, autoimmune diseases, allergy, and asthma, as well as tumor immunology.
Cytotoxic T cells isolated by MACS® Technology remain viable and functional. Therefore, they can be used for further functional studies, such as proliferation assays
2,9
and cytotoxicity assays
2,3
, but also for the analysis of
in vitro
cytokine production
4
. CD8 MicroBeads have also been used for the depletion of CD8
+
T cells from human PBMCs for immune reconstitution experiments in SCID mice.
5
In combination with CD4 MultiSort MicroBeads, CD8 MicroBeads have been used for the isolation of CD4
+
CD8
+
double-positive thymocytes.
8
Other examples include various studies on viral infections, e.g.,
in vitro
infections of CD8
+
cells with HIV
6
, or monitoring of immune abnormalities in children of HIV-infected mothers
7
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD8 MicroBeads, human

Reviews for CD8 MicroBeads, human

Anti-Human CD8 Microbeads - A Consistent and Reliable Method to Positively Select CD8+ T Cells from PBMC

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CD8 MicroBeads, human (130-045-201)

Our lab routinely works with purified immune cell subsets from human peripheral blood mononuclear cells (PBMC). In order to obtain highly pure CD8+ T cells, as well as other immune cell types, we routinely employ magnetic separation with Miltenyi products for positive selection because of their ease of use and minimal optimization. We require highly pure CD8+ T cells due to the nature of our downstream analysis, and this product helps us achieve this goal.

MicroBeads For Purification Of Human CD8 Positive Cells

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CD8 MicroBeads, human (130-045-201)

The general aim of my research is to investigate the role of TCRs in the function of CTLs. For that we isolated TCR genes from T cell clones isolated from HIV patients and cloned them into lentiviral vectors to use for transduction of primary CD8 T cells isolated from naive patients. These transduced cells are then used for further study to evaluate their ability to recognize their cognate peptide and their killing capacity of infected cells. We selected this product because we had access to the AutoMACSpro, an automated machine used for cell separation compatible with these beads.

CD8 Microbeads Review

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CD8 MicroBeads, human (130-045-201)

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. We use purified CD8 T cells from donors with known HLA types, to transduce them with the desired lentiviral construct and test their in vitro activity against HIV infected cells.

CD8 Magnetic Bead Isolation

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CD8 MicroBeads, human (130-045-201)

Great reagent to use for positive selection of CD8+ cells

References for CD8 MicroBeads, human

Publications

  1. Akdis, M. et al. (1999)
    Skin homing (cutaneous lymphocyte-associated antigen-positive) CD8
    +
    T cells respond to superantigen and contribute to eosinophilia and IgE production in atopic dermatitis.
    J. Immunol. 163: 466-475
  2. Parra, E. et al. (1997)
    The role of B7-1 and LFA-3 in costimulation of CD8
    +
    T cells.
    J. Immunol. 158: 637-642
  3. Turner, J. and Dockrell, H M. (1996)
    Stimulation of human peripheral blood mononuclear cells with live
    Mycobacterium bovis
    BCG activates cytolytic CD8
    +
    T cells
    in vitro
    .
    Immunology 87: 339-342
  4. Akdis, A. C. et al. (1995) Cytokine immunotrapping: an assay to study the kinetics of production and consumption or degradation of human interferon-gamma. J. Immunol. Methods 182: 251-261
  5. Walker, W. and Gallagher, G. (1994)
    The
    in vivo
    production of specific human antibodies by vaccination of human-PBL-SCID mice.
    Immunology 83: 163-170
  6. Ennen, J. et al. (1994) CD8+ T lymphocytes of African green monkeys secrete an immunodeficiency virus-suppressing lymphokine. Proc. Natl. Acad. Sci. U.S.A. 91: 7207-7211
  7. Nielsen, S. D. et al. (2001) Impaired progenitor cell function in HIV-negative infants of HIV-positive mothers results in decreased thymic output and low CD4 counts. Blood 98: 398-404
  8. Kutlesa, S. et al. (2002) Developmentally regulated interactions of human thymocytes with different laminin isoforms. Immunology 105: 407-418
  9. Al-Shanti, N. et al. (2004)
    Investigation of alpha nascent polypeptide-associated complex functions in a human CD8
    +
    T cell
    ex vivo
    expansion model using antisense oligonucleotides.
    Immunology 112: 397-403

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