Clone REAL215 is an antibody fragment derived from the full CD44 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity
Clone REAL215 recognizes the mouse CD44 antigen, which is also known as Pgp-1, HCELL, HUTCH-I, HCAM, HERMES, and Ly-24. CD44 is a 80–95 kDa membrane glycoprotein and is expressed by a variety of hematopoetic and nonhematopoetic cells. Different isoforms are generated by alternative splicing. Clone REA664 reacts with all isoforms and both allelic forms of CD44. CD44 functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). The interaction between Galectin-9 and CD44 increases the stability and function of adaptive regulatory T cells. CD44 expression is upregulated on naive T cells upon activation and high CD44 expression is maintained on memory T cells. Therefore, CD44 is often used to discriminate CD44hi antigen-experienced T cells from CD44low (CD62L
+) naive T cells.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).
