CD4
⁺
Recent Thymic Emigrant Isolation Kit
, human

Name: CD4+ Recent Thymic Emigrant Isolation Kit, human
Availability: InStock

CD4
⁺
Recent Thymic Emigrant Isolation Kit
, human

The CD4

⁺

Recent Thymic Emigrant Isolation Kit was developed for the isolation of CD4

⁺

CD45RA

⁺

CD31

⁺

recent thymic emigrants (RTE) cells from human PBMCs in a two-step procedure.

Product data and images for CD4
⁺
Recent Thymic Emigrant Isolation Kit
, human

Figures

Figure 1

Isolation of CD4

⁺

RTEs from PBMCs using the CD4

⁺

Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4

⁺

RTEs, PBMCs before separation and the isolated CD4

⁺

RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.

PBMCs before separation	PBMCs before separation gated on CD4 ⁺ CD45RA ⁺ cells
View details CD4 ⁺ Recent Thymic Emigrant Isolation Kit , human Figure 1 Isolation of CD4 ⁺ RTEs from PBMCs using the CD4 ⁺ Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4 ⁺ RTEs, PBMCs before separation and the isolated CD4 ⁺ RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.	View details CD4 ⁺ Recent Thymic Emigrant Isolation Kit , human Figure 1 Isolation of CD4 ⁺ RTEs from PBMCs using the CD4 ⁺ Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4 ⁺ RTEs, PBMCs before separation and the isolated CD4 ⁺ RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.
Isolated CD4 ⁺ RTEs	CD4 ⁺ RTEs gated on CD4 ⁺ CD45RA ⁺ cells
View details CD4 ⁺ Recent Thymic Emigrant Isolation Kit , human Figure 1 Isolation of CD4 ⁺ RTEs from PBMCs using the CD4 ⁺ Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4 ⁺ RTEs, PBMCs before separation and the isolated CD4 ⁺ RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.	View details CD4 ⁺ Recent Thymic Emigrant Isolation Kit , human Figure 1 Isolation of CD4 ⁺ RTEs from PBMCs using the CD4 ⁺ Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4 ⁺ RTEs, PBMCs before separation and the isolated CD4 ⁺ RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.

Specifications for CD4
⁺
Recent Thymic Emigrant Isolation Kit
, human

Overview

The CD4

⁺

Recent Thymic Emigrant Isolation Kit was developed for the isolation of CD4

⁺

CD45RA

⁺

CD31

⁺

recent thymic emigrants (RTE) cells from human PBMCs in a two-step procedure.

Detailed product information

Background information

RTEs are a subpopulation of naive T cells characterized by their content of T cell receptor excision circles (TRECs).

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) has been described as a marker to identify the CD4

⁺

RTEs among the naive CD4

⁺

T cell population, as its expression correlates with the TREC content of CD4

⁺

T cells

¹

. CD4

⁺

T cells lacking CD31 expression harbor little or no TRECs, suggesting that they already proliferated in the periphery.

Therefore, the magnetic isolation of CD4

⁺

RTEs is possible by direct isolation of CD31

⁺

cells from pre-enriched, untouched naive CD4

⁺

T cells.

Detailed separation procedure

The isolation is performed in a two-step procedure. First, non-CD4

⁺

and memory CD4

⁺

T cells are indirectly magnetically labeled with a cocktail of biotinylated antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted over a MACS

^®

Column. In the second step, the flow-through fraction of pre-enriched naive CD4

⁺

T cells is labeled with CD31 MicroBeads for subsequent positive selection of CD4

⁺

CD45RA

⁺

CD31

⁺

RTEs.

Columns

For the first magnetic separation (depletion): LS or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

References for CD4
⁺
Recent Thymic Emigrant Isolation Kit
, human

Publications

Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794

Data and images